. PCT PRINCIPLE : Pressure Cycling Technology (PCT) uses cycles of high and low pressure to break cells.
Video PCT (Pressure Bioscience) : http://www.pressurebiosciences.com/videos/video.html.
Use of PCT to break Legionnella cells : In the following study (see the poster at the link below) Pressure Bioscience Barocycler (model NEP3229) was used to break Legionella pneumophila Lens cells. After breakage by alternative cycles of high (30 000 psi) and low pressure the supension was subjected to RNA extraction and purification.
Poster : http://www.pressurebiosciences.com/downloads/3rdparty-2007-12/Poster-Barocycler.pdf

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